Achievements
CDRI, Lucknow
- Houses animals belonging to 25 different strains of 8 species of rodents, including 8 inbred strains of mice, 3 inbred strains of rat, two inbred strains of hamster, two strains of logomarphs and a strains of nude mice.
- Possesses SPF and complete germfree (gnotobiotic) rodent breeding nuclei and a facility for primate Behavior Studies and Primate Breeding.
- Tissue Culture Unit where human and animal cell lines are maintained and supplied. Enlarge out breeding colonies of present stock and establish new special strains. Develop animal stocks for experimentation, which can fulfill GLP standards. Increase stock of SPF and gnotobotic units. Develop human resource and provide consultancy in this area.
- A total number of 120000 laboratory animals were supplied for in house users and outside CPCSEA registered agencies/research institute for experimental use.
- Information/consultancy on various aspects of husbandry nutrition, genetics, microbiology and regarding design and construction of animal house were provided to a number of scientists several animal users of this as well as different institutes
NIN, Hyderabad
- A total number of more than 5 lakh animals supplied to different research institutions.
- A total of about 30,000 Kgs of animal feed for mice, rats, hamsters, guinea, pigs, rabbits and monkeys was supplied.
- The centre also provided technical consultancy on various aspects of laboratory animal care, breeding, nutrition, procumbent, animal experimental large and ethical aspects.
- Seven laboratory animal technician’s training course conducted and many persons were trained.
- The molecular analysis of obese rat developed and bred has become interesting model for work on nutrition and diabetes . Patent has been filed for the same
Centre for Genetic Engineering and Strain Manipulation, MKU, Madurai
Objectives
The manipulation of bacterial genomes with a focus on strain improvement is the main objective of this national facility. The metabolic events targeted for improvement are enzyme production and secondary metabolic production. Streptomyces being a prolific producer of commercially useful antibiotics was chosen. The continued use of E.coli compelled the continuation of the genome analysis in this organism also. Leprosy being a major concern in India, the genome analysis is M. leprae, the causative organism of Leprosy and Immunology of Leprosy is also actively pursued.
Achievements
- A chitinase overproducing mutant isolated in the center is developed for commercial production of adriamycin in chitin containing media, a cheaper alternative to conventional fermentation media. It was later shown that chitinase production in antibiotic producing S. peucetius is regulated by a two component regulatory system. A novel extracellular protein was also discovered binding daunorubicin
- Development and validation of an effective intergeneric conjugation system to insert foreign genes in a specific locus in Streptomyces producing adriamycin is completed.
- Discovered the condition under which the linear chromosome of Streptomyces converts itself into a circular chromosome. This conversion has profound effect on the stability of the Streptomyces strains.
- Developed a simple approach based on PCR to analyse the cytokine profiles in leprosy lesions. A sensitive approach was developed for mining expressed genes of M.leprae in infected biopsy samples. Comparative genomics of field isolated M.leprae was initiated.
- Contract research projects with industry in health care enzymes, vaccines and skin genes
- Service faciltities included DNA sequencing, real –time PCR and proteomics
International Depository Authority at Institute of Microbial Technology, Chandigarh
Objectives
Establishment of International Depository Authority Under Budapest treaty by upgrading the existing Microbial Type Culture Collection (MTCC) Facility at Institute of Microbial Technology, Chandigarh.
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